EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of adipocyte differentiation by PEGylated all-trans retinoic acid: reduced cytotoxicity and attenuated lipid accumulation



Regulation of adipocyte differentiation by PEGylated all-trans retinoic acid: reduced cytotoxicity and attenuated lipid accumulation



Journal of Nutritional Biochemistry 18(5): 322-331



Obesity is major risk factor for many disorders, including diabetes, hypertension and heart disease. Unfortunately, there is a dearth of therapeutic agents available to clinicians for the treatment of obesity. The principal aim of this study was to investigate whether PEGylated all-trans retinoic acid (PRA) can have favorable stability and biological activity in 3T3-L1 preadipocytes as an antiobesity drug. Here, we found that PRA inhibits the process of adipogenesis, including survival of adipocytes and differentiation to mature adipocytes. The results showed that RA nanoparticles (NPs) were prepared by PEGylation; below 200 nm, PRA-NPs were obtained. Moreover, PRA decreased glycerol-3-phosphate dehydrogenase activity in 3T3-L1 preadipocytes by acting with major adipocyte marker proteins such as PPAR gamma 2, C/ EBP alpha and aP2 modulators. Apoptosis, in addition, increased as the level of RA increased from 10 to 20 mu M, whereas PRA reduced apoptosis with increasing concentrations. Our data suggest that PRA-NP has potential as an antiobesity drug carrier due to its small particle size and PEGylated core-shell structure. In addition, our results suggest that PRA inhibits the process of adipogenesis and may be developed to treat obesity. Based on these results, PRA is suitable for adipocyte studies, and an enhanced effect of PRA with adipocyte differentiation offers a challenging approach for pharmaceutical applications.

(PDF emailed within 0-6 h: $19.90)

Accession: 013265027

Download citation: RISBibTeXText

PMID: 16963253

DOI: 10.1016/j.jnutbio.2006.06.004



Related references

Regulation of adipocyte differentiation by PEGylated all-trans retinoic acid: reduced cytotoxicity and attenuated lipid accumulation (vol 18, pg 322, 2007). 2008

RARĪ³-C-Fos-PPARĪ³2 signaling rather than ROS generation is critical for all-trans retinoic acid-inhibited adipocyte differentiation. Biochimie 106: 121-130, 2015

Regulation of keratinocyte proliferation and differentiation by all-trans-retinoic acid, 9-cis-retinoic acid and 1,25-dihydroxy vitamin D-3. Archives of Dermatological Research 288(12): 729-738, 1996

Novel retinoic acid, 9-CIS retinoic acid, in combination with all-trans retinoic acid is effective inducer of differentiation of retinoic acid resistant Hl 60 cells Relation to retinoic acid receptor and retinoid X receptor expression. Blood 82(10 SUPPL 1): 251A, 1993

Novel retinoic acid, 9-cis retinoic acid, in combination with all-trans retinoic acid is an effective inducer of differentiation of retinoic acid-resistant HL-60 cells. Blood 83(11): 3289-3297, 1994

Quantitative high-performance liquid chromatographic determination of retinoids in human serum using on-line solid-phase extraction and column switching: Determination of 9-cis-retinoic acid, 13-cis-retinoic acid, all-trans-retinoic acid, 4-oxo-all-trans-retinoic acid and 4-oxo-13-cis-retinoic acid. Journal of Chromatography B 691(1): 43-58, 1997

Liarozole-fumarate potentiates all-trans-retinoic acid-induced differentiation in in vitro and in vivo models through inhibition of the all-trans-retinoic acid catabolism. Anticancer Research 17(5C): 3975, 1997

Matrine cooperates with all-trans retinoic acid on differentiation induction of all-trans retinoic acid-resistant acute promyelocytic leukemia cells (NB4-LR1): possible mechanisms. Planta Medica 80(5): 399-408, 2014

Knockdown of XAB2 enhances all-trans retinoic acid-induced cellular differentiation in all-trans retinoic acid-sensitive and -resistant cancer cells. Cancer Research 67(3): 1019-1029, 2007

All-trans retinoic Acid differentiation syndrome chorioretinopathy: a case of multifocal serous neurosensory detachments in a patient with acute promyelocytic leukemia treated with all-trans retinoic Acid. Retinal Cases & Brief Reports 7(1): 46-49, 2013

All-trans-retinoic acid increases cytosine arabinoside cytotoxicity in HL-60 human leukemia cells in spite of decreased cellular ara-CTP accumulation. Annals of Oncology 10(3): 335-338, 1999

Counteraction of retinoic acid and 1,25-dihydroxyvitamin D3 on up-regulation of adipocyte differentiation with PPARc ligand, an antidiabetic thiazolidinedione, in 3T3-L1 cells. Life Sciences 62(14): 205-PL-211, 1998

Counteraction of retinoic acid and 1,25-dihydroxyvitamin D3 on up-regulation of adipocyte differentiation with PPARgamma ligand, and antidiabetic thiazolidinedione, in 3T3-L1 cells. Life Sciences 62(14): 205-211, Feb 27, 1998