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Transgenic plants expressing a single-chain Fv antibody to Tomato spotted wilt virus (TSWV) are resistant to TSWV systemic infection



Transgenic plants expressing a single-chain Fv antibody to Tomato spotted wilt virus (TSWV) are resistant to TSWV systemic infection



Acta Horticulturae (722): 337-348



Hybridoma cell lines secreting monoclonal antibodies to Tomato spotted wilt virus (TSWV) were characterized and one cell line was selected for cloning of the antibody genes. Universal degenerate oligonucleotides were designed to prime PCR amplification of the variable regions of the heavy and light chains of a monoclonal antibody. The PCR products were ligated to produce one single chain variable fragment (scFv) antibody gene construct that was used to transform Nicotiana tabacum Turk.

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