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A high-performance liquid chromatographic method for saikosaponin a quantification in rat plasma


A high-performance liquid chromatographic method for saikosaponin a quantification in rat plasma



Biomedical Chromatography: Bmc 21(5): 458-462



ISSN/ISBN: 0269-3879

PMID: 17370292

DOI: 10.1002/bmc.773

A high-performance liquid chromatographic method with UV detection has been developed for the determination of saikosaponin a in rat plasma. Saikosaponin a and internal standard jujuboside A were isolated from plasma samples by solid-phase extraction. The chromatographic separation was achieved on a reversed-phase C(18) column with the mobile phase of acetonitrile-water (35:65, v/v) at a flow rate of 1 mL/min and UV detection was set at 205 nm. The standard curve for saikosaponin a was linear over the concentration range 0.25-10 microg/mL and the limit of detection was 0.05 microg/mL. The absolute recovery was greater than 82%. The precision and accuracy ranged from 3.05 to 9.59% and 95.61 to 110.00%, respectively. The validated method was used to determine saikosaponin a in plasma samples in a pharmacokinetic study of saikosaponin a administered to Sprague-Dawley rats.

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Accession: 013616531

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