EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Cellular identification of a novel uncultured marine stramenopile (MAST-12 Clade) small-subunit rRNA gene sequence from a norwegian estuary by use of fluorescence in situ hybridization-scanning electron microscopy


Applied and Environmental Microbiology 73(8): 2718-2726
Cellular identification of a novel uncultured marine stramenopile (MAST-12 Clade) small-subunit rRNA gene sequence from a norwegian estuary by use of fluorescence in situ hybridization-scanning electron microscopy
Revealing the cellular identity of organisms behind environmental eukaryote rRNA gene sequences is a major objective in microbial diversity research. We sampled an estuarine oxygen-depleted microbial mat in southwestern Norway and retrieved an 18S rRNA gene signature that branches in the MAST-12 clade, an environmental marine stramenopile clade. Detailed phylogenetic analyses revealed that MAST-12 branches among the heterotrophic stramenopiles as a sister of the free-living Bicosoecida and the parasitic genus Blastocystis. Specific sequence signatures confirmed a relationship to these two groups while excluding direct assignment. We designed a specific oligonucleoticle probe for the target sequence and detected the corresponding organism in incubation samples using fluorescence in situ hybridization (FISH). Using the combined FISH-scanning electron microscopy approach (T. Stoeck, W. H. Fowle, and S. S. Epstein, Appl. Environ. Microbiol. 69:6856-6863, 2003), we determined the morphotype of the target organism among the very diverse possible morphologies of the heterotrophic stramenopilles. The unpigmented cell is spherical and about 5 [mu]m in diameter and possesses a short flagellum and a long flagellum, both emanating anteriorly. The long flagellum bears mastigonemes in a characteristic arrangement, and its length (30 [mu]m) distinguishes the target organism from other recognized heterotrophic stramenopiles. The short flagellum is naked and often directed posteriorly. The organism possesses neither a lorica nor a stalk. The morphological characteristics that we discovered should help isolate a representative of a novel stramenopile group, possibly at a high taxonomic level, in order to study its ultrastructure, physiological capabilities, and ecological role in the environment.


Accession: 013657337

PMID: 17293516

DOI: 10.1128/AEM.02158-06



Related references

In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization. European Journal of Oral Sciences 115(6): 459-467, 2007

Solenicola setigera is the first characterized member of the abundant and cosmopolitan uncultured marine stramenopile group MAST-3. Environmental Microbiology 13(1): 193-202, 2011

Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Applied and Environmental Microbiology 66(12): 5116-5122, 2000

The small subunit rRNA gene sequence of the chonotrich Chilodochona carcini Jankowski, 1973 confirms chonotrichs as a dysteriid-derived clade (Phyllopharyngea, Ciliophora). International Journal of Systematic and Evolutionary Microbiology 66(8): 2959-2964, 2016

Identification of bovine Neospora parasites by PCR amplification and specific small-subunit rRNA sequence probe hybridization. Journal of Clinical Microbiology 34(5): 1203-1208, 1996

Cellular identity of a novel small subunit rDNA sequence clade of apicomplexans: description of the marine parasite Rhytidocystis polygordiae n. sp. (host: Polygordius sp., Polychaeta). Journal of Eukaryotic Microbiology 53(4): 280-291, 2006

Fluorescence in situ hybridization using 16S rRNA-targeted oligonucleotides reveals localization of methanogens and selected uncultured bacteria in mesophilic and thermophilic sludge granules. Applied and Environmental Microbiology 65(3): 1280-1288, 1999

Scanning electron microscopy 1977 vol 1 proceedings of the 10th annual scanning electron microscope symposium and workshops on materials and component characterization quality control with the scanning electron microscopy scanning transmission electron microscopy scanning electron microscopy application to semi conductors analytical electron microscopy biological specimen preparation for scanning electron microscopy chicago illinois usa march 28 april 1 1977. Scanning Electron Microscopy 784, 1977

Detection of nucleolar organizer regions on chromosomes by fluorescence in situ hybridization with human 28S rRNA gene and cloning of 28S rRNA gene in Sika deer. Animal Science Journal 75(2): 111-116, 2004

Identification of aneuploids in uncultured amniotic fluid cells by interphase fluorescence in situ hybridization. Rinsho Byori. Japanese Journal of Clinical Pathology 46(5): 486-492, 1998