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Chemical method for estimating vitamin B12 in liver extracts



Chemical method for estimating vitamin B12 in liver extracts



Boll. Soc. ital. Biol. sper, 29: 471-473



The chief steps in the estimation were transformation of all forms of vitamin B12 to cyanocobalamin by treatment with KCN, removal of impurities by means of butanol and a saturated solution of (NH4)2SO4, extraction with water, reduction to dryness, ashing and, after other treatments including addition of alpha -nitroso- beta -naphthol, estimation by spectrophotometry.

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Accession: 013660258

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