+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Crystal structure of the yeast nicotinamidase Pnc1p



Crystal structure of the yeast nicotinamidase Pnc1p



Archives of Biochemistry and Biophysics 461(1): 66-75



The yeast nicotinamidase Pnc1p acts in transcriptional silencing by reducing levels of nicotinamide, an inhibitor of the histone deacetylase Sir2p. The Pnc1p structure was determined at 2.9A resolution using MAD and MIRAS phasing methods after inadvertent crystallization during the pursuit of the structure of histidine-tagged yeast isocitrate dehydrogenase (IDH). Pnc1p displays a cluster of surface histidine residues likely responsible for its co-fractionation with IDH from Ni(2+)-coupled chromatography resins. Researchers expressing histidine-tagged proteins in yeast should be aware of the propensity of Pnc1p to crystallize, even when overwhelmed in concentration by the protein of interest. The protein assembles into extended helical arrays interwoven to form an unusually robust, yet porous superstructure. Comparison of the Pnc1p structure with those of three homologous bacterial proteins reveals a common core fold punctuated by amino acid insertions unique to each protein. These insertions mediate the self-interactions that define the distinct higher order oligomeric states attained by these molecules. Pnc1p also acts on pyrazinamide, a substrate analog converted by the nicotinamidase from Mycobacterium tuberculosis into a product toxic to that organism. However, we find no evidence for detrimental effects of the drug on yeast cell growth.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 013675650

Download citation: RISBibTeXText

PMID: 17382284

DOI: 10.1016/j.abb.2007.01.037


Related references

A method to distinguish bovine origin tubercle bacillus in mycobacteria by nicotinamidase activity. Report I. Cell-free extract of nicotinamidase. Kekkaku 35: 287-291, 1960

Hydrolysis of nicotinyl hydroxamate by a yeast nicotinamidase. Archives of Biochemistry and Biophysics 120(1): 186-191, 1967

Hydrolytic enzyme of nicotinamide. I. Nicotinamidase of beer yeast. Jour Biochem [tokyo] 41(1): 89-99, 1954

Studies on hydrolytic enzyme of nicotinamide. 1. Nicotinamidase of beer yeast. J. Biochem., Tokyo. 41: 89-99, 1954

Studies on hydrolytic enzyme of nicotinamide. 2. Purification of nicotinamidase of beer yeast. J. Biochem., Tokyo. 41: 101-106, 1954

Structure of yeast hexokinase. 3. Low resolution structure of a second crystal form showing a different quaternary structure, heterologous interaction of subunits and substrate binding. Journal of Molecular Biology 86(2): 261-269, 1974

Structure of yeast hexo kinase part 3 low resolution structure of a 2nd crystal form showing a different quaternary structure heterologous interaction of subunits and substrate binding. Journal of Molecular Biology 86(2): 261-269, 1974

High-resolution crystal structures of Streptococcus pneumoniae nicotinamidase with trapped intermediates provide insights into the catalytic mechanism and inhibition by aldehydes. Biochemistry 49(40): 8803-8812, 2010

The crystal structure of yeast phenylalanine tRNA at 1.93 ANG resolution: A classic structure revisited. RNA (New York) 6(8): 1091-1105, 2000

Crystal structure of a yeast TBP. Nature 365(6446): 512-520, 1993

Determinants of enzymatic specificity in the Cys-Met-metabolism PLP-dependent enzymes family: crystal structure of cystathionine gamma-lyase from yeast and intrafamiliar structure comparison. Biological Chemistry 384(3): 373-386, 2003

Structure of yeast hexokinase. III. Low resolution struc ture of a second crystal form showing a different quaternary structure, heterologous interaction of subunits and substra te binding. Journal of molecular biology: 5, 86 (2) 261-269, 1974

Crystal structure of yeast Sco1. Journal of Biological Inorganic Chemistry 11(4): 459-466, 2006

Crystal structure of the 80S yeast ribosome. Current Opinion in Structural Biology 22(6): 759-767, 2012