Diversity and Phylogeny of rhizobia isolated from the nodules of broad bean (Vicia faba L.) in China

Lu-Minqi; Li-Jun; Jiang-Xin; Li-Li; Shen-Delong; Cao-Fengming

CHINESE JOURNAL OF APPLIED AND ENVIRONMENTAL BIOLOGY 13(1): 73-77

2007


ISSN/ISBN: 1006-687X
Accession: 013687327

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Abstract
Fifty Rhizobium strains, isolated from root nodules of broad bean (Vicaia faba L.) in 11 provinces of China were studied by numerical taxonomy, 16S rDNA PCR - RFLP, IGS PCR - RFLP and 16S rDNA full length sequence. The 50 strains were divided into four distinctive clusters at the similarity of 80% according to the numerical analysis. The result of 16S rDNA PCR - RFLP showed good agreement with that of numerical taxonomy. Compared with 16S rDNA PCR - RFLP, the result of IGS RFLP showed more differences among the strains. All the results demonstrated a high phenotypic and phylogenetic diversity of Rhizobsum sp. (V. faba L.). The full-length sequences of 16S rDNA of 5 representative strains were compared with those of some known rhizobia, and the result displayed that all the representative strains belonged to the same phylogenetic branch of Rhizobium.