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Effect of concentration of pantothenate on selection of a mutant for pantothenate synthesis in Saccharomyces cerevisiae

Effect of concentration of pantothenate on selection of a mutant for pantothenate synthesis in Saccharomyces cerevisiae

Genetics 35(3): 381-395

Adaptation of a haploid yeast from pantothenate dependence to independence was found to be due to mutation of a single gene. The mutation is not a back mutation to the dominant allele present in the pedigree studied, but is at a different locus, as identified by the independent segregation of the wild-type and the mutant synthesizer. Mutation to synthesis occurs by chance both in the presence and in the absence of pantothenate. However, in the presence of intermediate or high concns. of pantothenate, the nonsynthesizing cells overgrow the mutants and, therefore, adaptation occurs only in very low concns. of pantothenate. The dependent culture is able to grow slowly in the pantothenate-free medium after a long lag and thus is able to reach a population size such that mutation is likely to occur.

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Accession: 013691516

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PMID: 15414936

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