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Experimentally induced changes in the prothrombin level of the blood. 4. The relation of vitamin K deficiency to the intensity of dicumarol action and to the effect of excess vitamin A intake; with a simplified method for vitamin K assay



Experimentally induced changes in the prothrombin level of the blood. 4. The relation of vitamin K deficiency to the intensity of dicumarol action and to the effect of excess vitamin A intake; with a simplified method for vitamin K assay



J. Biol. Chem, 175: 945-952



A diet was devised which, in day-old chicks, within 10 days, produced a very low blood content of prothrombin and yet permitted growth. It contained per cent. casein 20, brewer's yeast 12, ground rice 67 and salts 1, with 1 ml. cod liver oil per 100 g. diet. The prothrombin time was determined in the blood by Quick's method (Amer. J. Clin. Path., 1940, 10, 222) with thromboplastin from chicken's brain. Comparison of the vitamin K potency of mena-dione, 2-methyl-1: 4-naphthaquinone, and of natural vitamin K1 was made in prophylactic tests on chicks receiving the deficient diet as above. Mena-dione maintained the prothrombin level in daily doses of from 1.5 to 2.0 micro g., while the corresponding amount of the natural vitamin was 2.5 micro g. With doses of menadione above 2 micro g., storage occurred. Either substance is considered suitable as standard of reference. In curative tests, the minimum effective dose of menadione was 5 micro g. Even when injected it did not act at once, and large doses did not act more rapidly than small ones. Chicks receiving the deficient diet were thereby rendered more susceptible to the action of dicou-marol, which could be counteracted by the administration of menadione or the natural vitamin. Excessive doses of vitamin A had no effect in increasing the requirement for vitamin K of chicks receiving the deficient diet.

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Accession: 013706296

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PMID: 18880791



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