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Frozen antigen for use in the complement fixation test for foot and mouth disease



Frozen antigen for use in the complement fixation test for foot and mouth disease



Gac. vet, B. Aires, 12: 105-117



Reduction of the anticomplementary power and relative increase in the titre of antigens is accomplished by freezing at -20 degrees C during 16-20 hours. Freezing at -20 degrees C is an excellent means of preserving already prepared antigens. Bovine tongue epithelium stored in phosphate buffer at pH 7.6 and 2.4 degrees C. maintains for years its complement fixing properties. Maceration of epithelium in pH 7.6 phosphate buffer at room temperature increases its anticomplementary action.

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Accession: 013717167

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