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Gravimetric estimation of choline and the lecithin, sphingomyelin and cephalin content of hen eggs and egg powders


Gravimetric estimation of choline and the lecithin, sphingomyelin and cephalin content of hen eggs and egg powders



Mitt. Geb. Lebensmittel. Hyg 44: 333-347



Total lipid is extracted by means of a mixture of ethanol and benzene and weighed. The extracted material is hydrolysed with HC1 in an autoclave and choline is precipitated as its reineckate and weighed. Lipid phosphoric acid and N can be estimated by application of saponification and Kjeldahl procedures, respectively, to the total lipid fraction. Prom these data the lecithin, cephalin and sphingomyelin content of egg powders can be calculated.

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Gravimetric method for determination of choline content as well as lecithin, sphingomyelin and cephalin in hen's eggs and powder eggs. Mitteilungen aus dem Gebiete der Lebensmittel-Untersuchung Un Hygiene 44(4): 333-347, 1953

The lecithin, cephalin, and sphingomyelin content of serum as indicated by the choline-phosphorus and nitrogen-phosphorus ratios. Journal of Biological Chemistry 174(1): 343-353, 1948

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