Improvement of biotechnological xylitol production by glucose during cultive of Candida guilliermondii in sugarcane bagasse hydrolysate
Virginio-Da-Silva, D.-Danielle; De-Mancilha, I.-Maciel; Da-Silva, S.-Silverio; De-Almeida-Felipe, M.-Das-Gracas
Brazilian Archives of Biology and Technology 50(2): 207-215
ISSN/ISBN: 1516-8913 DOI: 10.1590/s1516-89132007000200005
The effect of glucose on xylose-to-xylitol bioconversion by Candida guilliermondii was examined by adding it to sugarcane bagasse hydrolysate medium to obtain different glucose:xylose ratios (1:25, 1:12, 1:5 and 1:2.5). Under experimental conditions, increasing glucose:xylose ratio improved the assimilation of the xylose present in the hydrolysate by yeast, resulting in biomass increase, and in the formation of xylitol and glycerol/ethanol by-products. Maximum values of xylitol yield (0.59 g g(-1)) and volumetric productivity (0.53 g l(-1).h(-1)) were obtained with glucose:xylose ratio of 1:5, resulting in the higher conversion efficiency (64.3%).