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Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm



Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm



Infection and Immunity 75(6): 3192-3196



Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and (ii) show that anti-Acm immunoglobulin Gs (IgGs) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and 50%, respectively, significantly more than the total IgGs against the full-length Acm A domain (28%) (P < 0.0001). Blocking Acm adherence with functional subsegment-specific antibodies raises the possibility of their use as therapeutic or prophylactic agents.

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Accession: 013739731

Download citation: RISBibTeXText

PMID: 17438036

DOI: 10.1128/IAI.02016-06


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