+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Microarray analysis of homocysteine-responsive genes in cardiac neural crest cells in vitro

Microarray analysis of homocysteine-responsive genes in cardiac neural crest cells in vitro

Developmental Dynamics 236(4): 1044-1054

The amino acid homocysteine increases in the serum when there is insufficient folic acid or vitamin B(12), or with certain mutations in enzymes important in methionine metabolism. Elevated homocysteine is related to increased risk for cardiovascular and other diseases in adults and elevated maternal homocysteine increases the risk for certain congenital defects, especially those that result from abnormal development of the neural crest and neural tube. Experiments with the avian embryo model have shown that elevated homocysteine perturbs neural crest/neural tube migration in vitro and in vivo. Whereas there have been numerous studies of homocysteine-induced changes in gene expression in adult cells, there is no previous report of a homocysteine-responsive transcriptome in the embryonic neural crest. We treated neural crest cells in vitro with exogenous homocysteine in a protocol that induces significant changes in neural crest cell migration. We used microarray analysis and expression profiling to identify 65 transcripts of genes of known function that were altered by homocysteine. The largest set of effected genes (19) included those with a role in cell migration and adhesion. Other major groups were genes involved in metabolism (13); DNA/RNA interaction (11); cell proliferation/apoptosis (10); and transporter/receptor (6). Although the genes identified in this experiment were consistent with prior observations of the effect of homocysteine upon neural crest cell function, none had been identified previously as response to homocysteine in adult cells.

(PDF emailed within 0-6 h: $19.90)

Accession: 013759762

Download citation: RISBibTeXText

PMID: 17326132

DOI: 10.1002/dvdy.21101

Related references

Expression of trkC messenger RNA in avian cardiac neural crest cells and the effect of neurotrophin-3 on the number of cardiac neural crest cells in vitro. Society for Neuroscience Abstracts 25(1-2): 767, 1999

Functional analysis of cardiac neural crest genes by neural crest ablation and in ovo electroporation. FASEB Journal 18(4-5): Abst 535 6, 2004

Effect of elevated homocysteine on cardiac neural crest migration in vitro. Developmental Dynamics 224(2): 222-230, 2002

Homocysteine enhances cardiac neural crest cell attachment in vitro by increasing intracellular calcium levels. Developmental Dynamics 237(8): 2117-2128, 2008

Galpha-i signaling in neural crest cells is required for normal migration of cardiac neural crest cells, cardiac development, and survival. 2007

Migration and differentiation of neural crest and ventral neural tube cells in vitro: implications for in vitro and in vivo studies of the neural crest. Journal of Neuroscience 8(3): 1001-1015, 1988

Effects of Folic Acid and Homocysteine on the Morphogenesis of Mouse Cephalic Neural Crest Cells In Vitro. Cellular and Molecular Neurobiology (): -, 2016

Migration and differentiation of neural crest and non neural crest cells from quail neural tubes in vitro. Society for Neuroscience Abstracts 13(1): 699, 1987

Folate, homocysteine and the cardiac neural crest. Developmental Dynamics 242(3): 201-218, 2013

Transgenic expression of Cre recombinase in neural crest cells allows for fate mapping of cardiac neural crest in the developing mouse embryo and for tissue specific gene inactivation. Circulation 102(18 Supplement): II 98, October 31, 2000

Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells. Biology Open 5(3): 311-322, 2016

In vitro clonal analysis of quail cardiac neural crest development. Developmental Biology 148(1): 95-106, 1991

Microarray analysis of VEGF-responsive genes in myometrial endothelial cells. Molecular Human Reproduction 8(9): 855-863, 2002

Homocysteine inhibits cardiac neural crest cell formation and morphogenesis in vivo. Developmental Dynamics 229(1): 63-73, 2003

Microarray analysis of VEGF-C responsive genes in human lymphatic endothelial cells. Lymphatic Research and Biology 3(4): 183-207, 2005