+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Molecular characterization, expression and mapping of porcine LMP2 and MECL-1 genes

Molecular characterization, expression and mapping of porcine LMP2 and MECL-1 genes

Dna Sequence 18(4): 257-264

Low molecular mass polypeptides 2 (LMP2) and Multicatalytic endopeptidase complex subunit (MECL-1) are two of three catalytic beta-type subunits of the 20s proteasome and upon interferon gamma-induction. LMP2 is critical for the production of major histocompatibility complex (MHC) class I ligand and T-lymphocytes. The LMP2 gene is located in the MHC region, but MECL-1 is located outside the MHC region. They are involved in the antigen presentation and are important candidate genes for an initial exploration of relationships between the antigen processing genes and disease resistance. In this report, the porcine LMP2 and MECL-1 cDNA were cloned and A 5099 bp LMP2 genomic DNA structure was identified, then two single nucleotide polymorphisms were detected in the exon2 and exon5 of LMP2 gene in 367 individuals. The LMP2 and MECL-1 genes putative protein included 219,274 amino acids, respectively. Alignment and phylogenetic of predicted porcine LMP2 and MECL-1 amino acid sequence with their homologies were analyzed. Tissues expression of LMP2 and MECL-1 mRNA were observed by real time quantitative PCR (Q-PCR) method, the results revealed MECL-1 expressed widely in all tissues, but LMP2 was not detected in muscle. The porcine MECL-1 gene was mapped to chromosome 6, closely linked to microsatellite SW1108 (LOD = 4.09, 84cR) by radiation hybrid panel.

(PDF emailed within 0-6 h: $19.90)

Accession: 013763013

Download citation: RISBibTeXText

PMID: 17541830

DOI: 10.1080/10425170601119222

Related references

Anti-viral immune responses in a primitive lung: characterization and expression analysis of interferon-inducible immunoproteasome subunits LMP2, LMP7 and MECL-1 in a sarcopterygian fish, the Nigerian spotted lungfish (Protopterus dolloi). Developmental and Comparative Immunology 41(4): 657-665, 2014

Molecular characterization, mapping, and haplotype analysis of porcine matrix metalloproteinase genes MMP1 and MMP10. Biochemical Genetics 47(11-12): 763-774, 2011

Dextran sulfate sodium-induced colitis is associated with enhanced low molecular mass polypeptide 2 (LMP2) expression and is attenuated in LMP2 knockout mice. Digestive Diseases and Sciences 51(7): 1269-1276, 2006

The subunits MECL-1 and LMP2 are mutually required for incorporation into the 20S proteasome. Proceedings of the National Academy of Sciences of the United States of America 94(17): 8970-8975, 1997

Molecular characterization, chromosome mapping, and expression profile of porcine CDC16. Genetics and Molecular Research 14(4): 12811-9, 2016

Full-length cDNA, molecular characterization and physical mapping of five genes from a porcine fetal cDNA library. Animal Genetics 37(1): 82-84, 2006

Molecular characterization and expression patterns of the actinin-associated LIM protein (ALP) subfamily genes in porcine skeletal muscle. Gene 539(1): 111-116, 2014

Molecular characterization, expression profiles of the porcine SDC2 and HSPG2 genes and their association with hematologic parameters. Molecular Biology Reports 40(3): 2549-2556, 2014

Molecular characterization, expression profiles, and association analysis with hematologic parameters of the porcine HPSE and HPSE2 genes. Journal of Applied Genetics 54(1): 71-78, 2013

Molecular characterization of the encoding regions and tissue expression analyses for three novel porcine genes--HNRPA1, YIPF5 and UB2D2. Molecular Biology Reports 35(4): 519-526, 2007

Molecular characterization of the encoding regions and tissue expression analyses for three novel porcine genes - HNRPA1, YIPF5 and UB2D2. Molecular Biology Reports: 4, 519-526, 2008

Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28a/b, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope. Journal of Immunology 165(2): 8-78, 2000

Molecular cloning, characterization, and expression of multiple porcine cytochrome P450 aromatase complementary deoxyribonucleic acid and chromosomal genes. 1996

Association analysis of polymorphisms of porcine LMP2 and LMP7 genes with haematological traits. Molecular Biology Reports 38(7): 4455-4460, 2011