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Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens



Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens



Biological & Pharmaceutical Bulletin 30(4): 798-800



We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug efflux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.

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Accession: 013763023

Download citation: RISBibTeXText

PMID: 17409524

DOI: 10.1248/bpb.30.798



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