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Pantothenate studies. 1. Interferences by D-serine and L-aspartic acid with pantothenate synthesis in Escherichia coli



Pantothenate studies. 1. Interferences by D-serine and L-aspartic acid with pantothenate synthesis in Escherichia coli



J. Bacterial, 80: 733-745



Pantothenic acid was estimated with a mutant of Bact, coli which responded specifically to it, the medium used being that of Davis and Mingioli (J. Bacterial, 1950, 60, 7). Growth of the wild type of Bact. coli on a medium previously described (Title 123, Vol. 19) was inhibited by the addition of 50, 200 or 1000 micro g. D-serine per ml. The inhibition was completely reversed by addition of Ca pantothenate and less completely by beta -alanine. The concentration of beta -alanine required to reverse the inhibition was proportional to the concentration of D-serine, indicating that D-serine interfered with the conversion of beta -alanine to pantothenate. L-Aspartic acid increased the concentration of beta -alanine needed to counteract D-serine but by itself had no inhibitory effect. The presence of DL-serine delayed the appearance of colonies; addition of 30 beta g. Ca pantothenate per ml, completely reversed the effect of various concentrations of DL-serine but could not overcome the effect of a concentration as great as 1 mg. per ml., which apparently interfered with metabolic processes other than the synthesis of pantothenate. Growth experiments with mutants whose ability to synthesis beta -alanine was blocked confirmed the hypothesis that D-serine interfered with the conversion of beta -alanine to pantothenate. In resting cell suspensions which synthesise pantothenate in the presence of beta -alanine and excrete it into the medium, the synthesis was inhibited by presence of D-serine; a complete reversal could be obtained by a large increase in the beta alanine concentration. Evidence was obtained that the inhibiting substance was D-serine itself and not a conversion product of it. Pantoyl lactone, the other precursor moiety of pantothenate, was not needed for pantothenate production by resting cells though its addition markedly increased output.

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Accession: 013784883

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PMID: 14824065


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