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Photoreactivation Of Ultraviolet-Irradiated Escherichia Coli, With Special Reference To The Dose-Reduction Principle And To Ultraviolet-Induced Mutation

Photoreactivation Of Ultraviolet-Irradiated Escherichia Coli, With Special Reference To The Dose-Reduction Principle And To Ultraviolet-Induced Mutation

Journal of Bacteriology 58(4): 511-522

Visible light of wave lengths under 5, 100 A will cause the recovery of microbial cells from ultraviolet-induced injury which would otherwise be fatal. Light-induced recovery, or photo-reactivation, occurs in at least four diverse species, Escherichia coli B/r, Streptomyces griseta ATC 8826, Penicilliitm notation, and Saccharomyces cerevisiae. In E. coli B/r the ultraviolet dose-survival curves for suspensions kept dark after irradiation, and for suspensions photoreactivated, have in general similar shapes. From the similarity in shape of the curves there was evolved the dose-reduction principle-the effect of a constant amount of reactivating light on survival in a suspension iradiated with varying amounts of ultraviolet light is the same as if it decreased the effective lultraviolet dose by a constant factor. For E. coli B/r this means that the amount of reactivating light used in one experiment reduced the dose of ultraviolet light effective in killing cells by 60 per cent. E. coli B/r cells incubated in broth at 37C, in the dark, after ultraviolet irradiation, lost their ability to be photoreactivated. The ability to recover decreased exponentially with incubation time, becoming zero after 2 to 8 hours of incubation. Induced mutants occur in photoreactivated E. coli B/r cells. At the ultraviolet doses used, reactivating light apparently reduced the frequency of mutants characterized by phenotypic expression within a few minutes after irradiation (zero-point mutants) but had little or no effect on mutants characterized by phenotypic expression only after a prolonged period of incubation. The possible significance of these data in the light of the dose-reduction principle is discussed.

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Accession: 013789744

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PMID: 16561813

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