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Separation and determination of vitamin B6 group (pyridoxine, pyridoxal, pyridoxamine, pyridoxal-phosphate and pyridoxamine-phosphate); separation by paper electrophoresis of riboflavin (riboflavin, FMN and FAD) and of nicotinamide (nicotinamide, DPN and TPN) groups


Separation and determination of vitamin B6 group (pyridoxine, pyridoxal, pyridoxamine, pyridoxal-phosphate and pyridoxamine-phosphate); separation by paper electrophoresis of riboflavin (riboflavin, FMN and FAD) and of nicotinamide (nicotinamide, DPN and TPN) groups



Biochimica et Biophysica Acta 14(2): 212-218



ISSN/ISBN: 0006-3002

PMID: 13172238

Separation of the substances of the vitamin B6 group was by electrophoresis and spraying with diazotised p-aminoacetophenone. The colour of the spots ranged from pink to yellow, and was stable; there was no breakdown of the esters. Methods are described for making the estimation quantitative. Riboflavin and its coenzymes were separated by the same methods of electrophoresis on paper. The spots were located by their bright yellow fluorescence in ultraviolet light. Nicotinamide and its coenzymes also were separated; di- and triphosphopyridine nucleotides were converted to the dihydro-forms after electrophoresis, by spraying with Na2S2O3and NaHCO3, when their spots showed a feeble fluorescence in ultraviolet light; nicotinamide and its compounds showed dark spots. The three methods listed above could be applied to detection of all the substances in presence of one another. A " Mineral light " was used first; it showed all the spots and identified those due to riboflavin compounds by their yellow fluorescence; their specific reactions could then be applied to the vitamin B6and nicotinamide groups. V. R. Jackson.

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Accession: 013824266

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