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Studies on amoebiasis in Canada. Part II. A method for obtaining viable cysts of Entamoeba histolytica free from bacteria



Studies on amoebiasis in Canada. Part II. A method for obtaining viable cysts of Entamoeba histolytica free from bacteria



Canad. J. Res, 26: 299-302



In order to obtain the cysts free from bacteria the authors thoroughly washed faecal samples by means of centrifugation in water and then Ringer's solution. The cysts were then floated in zinc sulfate solution, picked off and again washed in sterile Ringer's. They were transferred by means of a micropipette to a solution of penicillin and streptomycin in sterile Ringer's solution, five transfers into fresh solutions of the antibiotics being made.

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