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Chapter 13,886

The microbiological assay of amino acids. I. The assay of tryptophan, leucine, isoleucine, valine, cystine, methionine, lysine, phenylalanine, histidine, arginine and threonine

Barton-Wright, E.C.

Analyst 71(843): 267-278

1946


ISSN/ISBN: 0003-2654
DOI: 10.1039/an9467100267
Accession: 013885899

Tryptophane, leucine, valine, isoleucine and cystine were assayed by Lactobacillus arabinosus 17/5; methionine, lysine, phenylalanine and histidine by Leuconostoc mesenterioides P-60; and arginine and threonine by Streptococcus faecalis (lactis) R. The natural L acids were active as growth factors, but the D forms were inactive. For all assays except tryptophane hydrolysates were prepared by autoclaving with 2.5N-HCl for 5 to 6 hr. at 15 Ib. pressure.

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