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The purification of a glucuronide of nicotinic acid from rat urine

The purification of a glucuronide of nicotinic acid from rat urine

Journal of Biological Chemistry 217(1): 287-292

Bioautographic studies on normal rat urine indicated the presence of a hitherto unidentified excretory product of nicotinic acid. The urine of rats fed the vitamin was used to isolate the substance. It was first precipitated as a lead salt and then purified on a Dowex 50 ion exchange column, with 0.1N HCl as eluent. Final purification was effected by paper chromatography. Various colorimetric, chromatographic, spectrophotometric, and microbiological tests demonstrated the presence of equimolar amounts of nicotinic acid and glucuronic acid in the molecule. Hydrolysis experiments with alkali and with beta-glucuronidase indicated that the new metabolite is beta-nicotinoyl-D-glucuronic acid. It is extremely labile to mild alkaline conditions. It was not detected in the normal urine of species other than the rat.

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Accession: 013892755

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PMID: 13271393

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