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Cloning, identification, sequence assembly and sequence analysis and application of part gene in capsular polysaccharide export gene of Actinobacillus pleuropneumoniae


Cloning, identification, sequence assembly and sequence analysis and application of part gene in capsular polysaccharide export gene of Actinobacillus pleuropneumoniae



Chinese Journal of Veterinary Science 27(3): 325-329



Different sequences of the capsular polysaccharide export (cpx) gene were amplified and cloned with primers from A. pleuropneumoniae serotypes 1, 3, 6 and 9. The complete sequence of the cpxD gene of serotypes 1, 3 and 6 were assembled by the bio-software based on cloned sequences and part sequences in GenBank. Sequence analysis showed that the homology among serotypes 1, 3, 5, 6 and 9 was between 89-99.88%.

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