Estimation of number of root-nodule bacteria by a nodulation-dilution frequency method. Ecological studies of Rhizobium in soils (Part 1)

Tuzimura, K.; Watanabe, I.

Soil Sci. & Plant Nutr 7: 61-65


Accession: 014037474

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Test tubes containing sand or vermiculite-sand moistened with N-free nutrient solution were sterilized and 2-3 aseptically germinated seeds sown in each. Normally prepared soil suspensions were used for inocula. Serial ten-fold dilutions were made up. The number of tubes for each dilution was 5 or 10. By applying Halvoron-Ziegier's table "most probable numbers" were calculated from the frequency of nodulated tubes for each dilution.