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Immunological studies on African swine fever virus. I. Elimination of the procomplementary activity of swine serum with formalin



Immunological studies on African swine fever virus. I. Elimination of the procomplementary activity of swine serum with formalin



Journal of Immunology 86: 465-470



For use in the complement fixation test for African swine fever, the procomplementary effect of swine serum was eliminated by adding 0.2 ml of a 1:72 dilution of formalin (37-41% formaldehyde solution) to 1.0 ml of heated (56[degree] C) swine serum and incubating for 45 minutes at 37[degree] C. This treatment abolished, or markedly reduced, the prozone effects usually obtained with swine serum in the complement fixation test. While serum titers were somewhat depressed by the formalin treatment, the procedure proved of value in that positive tests for antibody were obtained with low titered sera which were negative when tested untreated. This is attributed to the fact that elimination of the pro-complementary activity abolishes the prozone which masks any indication of a complement fixation reaction.

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Accession: 014073868

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PMID: 13696091



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