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Reciprocal transfer of cattle and rabbit embryos

Reciprocal transfer of cattle and rabbit embryos

Jour Animal Sci 22(1): 30-35

Embryos were recovered from superovulated rabbits and transferred to beef cattle. Three techniques of embryo transfer were attempted: (a) non-surgical, (b) non-surgical with CO2 insufflation, using the "Kiddie" insufflator and (c) surgical via a paralumbar laparotomy. An apparatus made of 3 plastic tubes and 2 long metal needles was used to by-pass the cervix and to transfer the embryos non-surgically into the uterus. Carbon dioxide insufflation through the cervix was essential for successful transfer. This non-surgical technique was inferior to the surgical technique in terms of percent of embryos successfully transferred. Viable rabbit embryos of 16- and 32-cell stages developed to the blastocyst stage in the cow's uterus. Embryos were recovered from superovulated cattle and transferred by laparotomy to the Fallopian tubes or uteri of 1-, 2-, 3- and 4-day pseudopregnant rabbits. Viable cattle embryos of 1- to 8-cell stages failed in most cases to cleave when kept for 48 hr. in the genitalia of pseudopregnant rabbits.

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Accession: 014163127

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DOI: 10.2527/jas1963.22130x

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