+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Recurrent changes in colony morphology dependent on intracolony selection of prototrophs occurring in red yeast

Recurrent changes in colony morphology dependent on intracolony selection of prototrophs occurring in red yeast

Heredity 12(2): 257-270

Under particular conditions of the growth medium, massive bursts of white, prototrophic cells arise spontaneously from colonies of certain strains of red, adenine-requiring yeasts (Saccharomyces cerevisiae). The occurrence of prototrophs is the result of back-mutation at the locus for adenine requirement. The "burst" phenomenon occurs when the pH of the medium is 4.5, but does not occur at pH levels of 5.0 and above. Well-developed bursts occur only when adenine concentration is partly limiting the growth of red cells. The burst phenomenon depends upon environmental conditions such that prototrophs have strong selective advantages within colonies of adenine-requiring cells, since (1) prototrophic cells can be shown to be present in colonies grown under conditions where bursts never arise; (2) prototrophic cells mechanically transferred into colonies of red yeast always proliferate into bursts if the colonies are grown on media that permit spontaneous occurrence of bursts; (3) transferred prototrophs never proliferate into bursts if the host red colonies are grown on media where spontaneous bursts never occur. Experiments with artificially constructed giant colonies show that any independently occurring prototrophic cell has a high probability of expressing itself as a burst, under the appropriate environmental conditions noted above. Some implications of the burst phenomenon for general problems in development are discussed.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 014163596

Download citation: RISBibTeXText

DOI: 10.1038/hdy.1958.27

Related references

Infra-colony selection of prototrophs occurring spontaneously in an adenine-requiring strain of yeast. Genetics. 42: 398 p, 1957

Intracolony variation in colony morphology in reassembled fossil ramose stenolaemate bryozoans from the Upper Ordovician (Katian) of the Cincinnati Arch region, USA. Journal of Paleontology 90(03): 400-412, 2016

Random colony selection versus colony morphology for detection of multiple pneumococcal serotypes in nasopharyngeal swabs. Pediatric Infectious Disease Journal 27(2): 178-180, 2008

Colony structure of a slavemaking ant. I. Intracolony relatedness, worker reproduction, and polydomy. Evolution 55(2): 307-315, 2001

Intracolony variability during periods of poor reproductive performance at a glaucous winged gull colony. Condor 94(3): 598-607, 1992

A selective system for identifying single cell prototrophs in intact asci of yeast. Genetics. 50: 247 p, 1964

A study of mutants occurring in the giant colony of a yeast strain isolated from wine. CR Acad. Sci, Paris, 252: 3321-23, 1961

Quantitative analysis of colony morphology in yeast. Biotechniques 56(1): 18-27, 2014

Environmental and genetic determinants of colony morphology in yeast. Plos Genetics 6(1): E1000823, 2010

Some innovations in rice recurrent selection: The back recurrent selection (BCRS), the simplified and efficient rice breeding method (SERB), and the plant-parasite reciprocal recurrent selection (2P2RS). Crop Protection 29(4): 311-317, 2010

Use of naturally occurring chitinase activity and colony morphology for monitoring epiphytic populations of applied antagonists. Phytopathology 82(10): 1178, 1992

Initial colony morphology-based selection for iPS cells derived from adult fibroblasts is substantially improved by temporary UTF1-based selection. Plos One 5(3): E9580, 2011

Mutational variability of colony and cell morphology in the yeast Saccharomyces cerevisiae. Soviet genetics (pub 1979) 14(9): 1085-1091, 1979

Mutational variability of colony and cell morphology in the yeast saccharomyces cerevisiae. Genetika 14(9): 1543-1551, 1978

Farnesol restores wild-type colony morphology to 96% of Candida albicans colony morphology variants recovered following treatment with mutagens. Genome 49(4): 346-353, 2006