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Red-blue staining of hydrolysed nucleic acids in paraffin sections

Red-blue staining of hydrolysed nucleic acids in paraffin sections

Stain Technology 38: 157-160

A previous treatment with 10% HC1 in tetrahydrofuran for 2-3 min at 37[degree] C hydrolyses DNA while substantially preserving RNA in formol-fixed paraffin sections. If this treatment is followed by dyeing with basic fuchsin-thiazine or oxazine mixtures, the basic fuchsin stains DNA, the blue dye cytoplasmic RNA, though nucleolar RNA is not well preserved. A specimen sequence is to treat the hydrolysed section with a mixture of 1% aqueous trimethylthionin (Chroma), 15 ml; 0.1% basic fuchsin (G. T. Gurr),4 ml; and glacial acetic acid, 1 ml. Stain for 15-30 min, dehydrate in acetone, then pass sections through xylene to polystyrene. The specificity of this stain for cytoplasmic RNA is sharper than that of methyl green-pyronin; hence the technic given can be a useful addition to the standard Unna-Pappenheim procedure.

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Accession: 014163810

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PMID: 13934908

DOI: 10.3109/10520296309067159

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