+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Studies on the industrial production of flavin-adenine dinucleotide. 2. Purification of flavin-adenine dinucleotide using ion-exchange resins



Studies on the industrial production of flavin-adenine dinucleotide. 2. Purification of flavin-adenine dinucleotide using ion-exchange resins



J. Vitaminol., Japan. 6: 77-85



Extraction of FAD from mycelia of E. ashbyii involved its separation from the free riboflavin which was present also. The most satisfactory large-scale procedure was found to be preliminary extraction with a mixture of pyridine, methanol and water, followed by adsorption on the strongly basic Amberlite resin IRA-401-Cl, elution with dilute NaCl solution, extraction first with phenol, then with water, and final precipitation with alcohol. Yield was 50% and purity 85%. A. M. Copping.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 014208707

Download citation: RISBibTeXText

PMID: 14406230


Related references

Studies on the industrial production of flavin-adenine dinucleotide. 1. Preparation of mycelium of Eremothecium ashbyii as a rawmaterial containing flavin-adenine dinucleotide. J. Vitaminol., Japan. 6: 68-76, 1960

Studies on the industrial production of flavinadenine dinucleotide. III. Purification of flavin-adenine dinucleotide using active carbon and by metallic salt formation. Journal of Vitaminology 6: 87-93, 1960

Selection of Microorganisms Producing Flavin-Adenine Dinucleotide from FMN and Adenine (AMP) and Production of Flavin-Adenine Dinucleotide by Sarcina lutea. Agricultural and Biological Chemistry 37(4): 849-856, 1973

Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. V. Studies with the Escherichia coli hemoflavoprotein depleted of flavin mononucleotide: distinct roles for the flavin adenine dinucleotide and flavin mononucleotide prosthetic groups in catalysis. Journal of Biological Chemistry 249(5): 1599-1609, 1974

Purification of flavin mononucleotide-dependent and flavin-adenine dinucleotide-dependent reduced nicotinamide-adenine dinucleotide phosphate-cytochrome P-450 reductase by high-performance liquid chromatography on hydroxyapatite. Journal of Chromatography 358(1): 296-301, 1986

Initial characterization of the primary photochemistry of AppA, a blue-light-using flavin adenine dinucleotide-domain containing transcriptional antirepressor protein from Rhodobacter sphaeroides: a key role for reversible intramolecular proton transfer from the flavin adenine dinucleotide chromophore to a conserved tyrosine?. Photochemistry and Photobiology 78(3): 290-297, 2003

Modular engineering of the flavin pathway in Escherichia coli for improved flavin mononucleotide and flavin adenine dinucleotide production. Journal of Agricultural and Food Chemistry 2019, 2019

Site-specific immobilization of flavin adenine dinucleotide on indium/tin oxide electrodes through flavin adenine amino group. Applied Biochemistry and Biotechnology 11(3): 221-232, 1985

Organic Phosphates. IX. An Effective Separation of Flavin Nucleotides and Adenine Nucleotide from Hydrolysates of Flavin Adenine Dinucleotide. Chemical & Pharmaceutical Bulletin 8(5): 436-440, 1960

Effect of administration of vitamin A, ascorbic acid and nicotinamide adenine dinucleotide + flavin adenine dinucleotide on severity of myocardial infarction induced by isoproterenol in rats. Indian Journal of Experimental Biology 23(9): 500-504, 1985

The flavoprotein domain of P450BM-3: expression, purification, and properties of the flavin adenine dinucleotide- and flavin mononucleotide-binding subdomains. Biochemistry 35(23): 7528-7535, 1996

Electrochemical determination of nicotinamide adenine dinucleotide and hydrogen peroxide based on poly(xanthurenic acid), flavin adenine dinucleotide and functionalized multi-walled carbon nanotubes. Sensors and Actuators B: Chemical 184: 212-219, 2013

Studies on flavin adenine dinucleotide biosynthesis using P32-labeled flavin mononucleotide. Journal of Vitaminology 2(1): 57-59, 1956

Isolation and identification of flavin adenine dinucleotide. II. Studies on purification. Journal of Vitaminology 1(3): 184-192, 1955

The flavoprotein component of the Escherichia coli sulfite reductase: expression, purification, and spectral and catalytic properties of a monomeric form containing both the flavin adenine dinucleotide and the flavin mononucleotide cofactors. Biochemistry 37(17): 6114-6123, 1998