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Studies on the industrial production of flavin-adenine dinucleotide. 2. Purification of flavin-adenine dinucleotide using ion-exchange resins

Studies on the industrial production of flavin-adenine dinucleotide. 2. Purification of flavin-adenine dinucleotide using ion-exchange resins

J. Vitaminol., Japan. 6: 77-85

Extraction of FAD from mycelia of E. ashbyii involved its separation from the free riboflavin which was present also. The most satisfactory large-scale procedure was found to be preliminary extraction with a mixture of pyridine, methanol and water, followed by adsorption on the strongly basic Amberlite resin IRA-401-Cl, elution with dilute NaCl solution, extraction first with phenol, then with water, and final precipitation with alcohol. Yield was 50% and purity 85%. A. M. Copping.

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Accession: 014208707

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PMID: 14406230

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