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A new overlay for plaquing animal viruses

Mirchamsy, H.; Rapp, F.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 129(1): 13-17

1968


ISSN/ISBN: 0037-9727
PMID: 4301087
DOI: 10.3181/00379727-129-33237
Accession: 014316489

An overlay employing tragacanth gum as a substitute for agar in the plaque assay of animal viruses is described. The tragacanth overlay was successfully employed with the vesicular stomatitis, F & M disease, pseudorabies, African horse sickness, and measles viruses. Plaques under tragacanth were generally smaller but the counts usually equalled or slightly exceeded those obtained under agar.

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