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Blood amino acid studies: prediction of limiting amino acids in mixtures of dietary proteins



Blood amino acid studies: prediction of limiting amino acids in mixtures of dietary proteins



Canad. J. Physiol. Pharmacol 46: 707-711



Weanling male rats were given for 5 days, with food available for 18 h each day, diets with 9% protein of which two-thirds was from a cereal and one-third from a protein supplement. Blood was obtained from fed rats, 6 h after the day's food was supplied, or from fasting rats when food was withheld for 16 h. Plasma concentrations of lysine, methionine, threonine, tryptophan, leucine and isoleucine were estimated and PAA ratios (Longenecker and Hause, Abst. 2280, Vol. 30) and PAA scores (McLaughlan, Abst. 2193, Vol. 35) were calculated. The free amino acids in plasma were estimated microbiologically. Chemical scores of the diets and protein efficiency ratios (PER) were estimated also. PAA ratios, PAA scores and PER indicated that lysine and threonine were equally limiting in mixtures of rice with groundnut, rice with soya bean, rice with cottonseed, rice with chickpea, wheat with groundnut and wheat with cottonseed proteins. The plasma values also showed that lysine was limiting in mixtures of maize with groundnut, soya or cottonseed protein. Plasma values did not indicate a methionine deficiency when methionine was co-limiting with lysine and threonine. Chemical score failed to detect the threonine deficiency present in 8 of 12 diets, which required threonine as well as lysine. It was concluded that the PAA methods are suitable for predicting a deficiency of a single amino acid or of two limiting amino acids, but are less satisfactory for predicting multiple deficiencies.

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Accession: 014363418

Download citation: RISBibTeXText

PMID: 5681531

DOI: 10.1139/y68-111


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