An experimental study has been carried out to investigate the effect of intermittent starvation followed by normal feeds with and without a fat supplement (butter fat) on blood and tissue lipids of rabbits. Although a significant decrease of plasma /3-lipoprotein percent is noted in animals subjected to periodic starvation and refeeding of diet, the other blood parameters do not show any marked alteration in the various groups. Estimation of tissue lipids indicates a disturbance of the biosynthesis of fats in periodically starved animals. There is a marked increase of cholesterol/phospho-lipid ratio in all the tissues when animals are alternately fed fat after periods of starvation but a decrease is noted when normal feed replaces the fat. The possible significance of these changes in the production of vascular pathology has been discussed.