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Carbon dioxide in anaerobic spermatozoan metabolism

Carbon dioxide in anaerobic spermatozoan metabolism

Journal of Dairy Science 51(1): 96-103

The mechanism of CO2 effects in mammalian spermatozoan metabolism is little understood. The demonstration of a need for CO2 in anaerobic metabolism ofepididymal-like bovine spermatozoa pre-sentedan opportunity for further study of CO2 effects on glycolysis. CO2 evolution, substrate disappearance, and end product accumulation during 4 hr. incubation at 37[degree]C were the criteria of measurement. A PCO2 of 2 to 3 % was optimum for maximum glycolysis, while levels above and below this narrow optimum range progressively depressed glycolysis. An initial PCO2 of 1% or greater maintained spermatozoan motility, but lower levels did not. Sperm cells deprived of CO2 for 2 hr. or longer did not recover metabolism or motility when regassed with 5% CO2 95% N2, whereas cells first subjected to 5% CO2 demonstrated inhibited metabolism resulting from the removal of CO2 only after the first hours. With fructose as substrate, a study of the addition of several substances revealed that only the complex material egg yolk replaced CO2 in initiating glycolysis. CO2 was necessary for the anaerobic cellular conversion of pyruvate to lactate. The addition of NADH caused an early, marked uptake of CO2, which could be completely inhibited by [gamma]-(3,4-urelenecyclohexyl) butyric acid, a biotin antagonist.

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Accession: 014370933

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PMID: 5689108

DOI: 10.3168/jds.S0022-0302(68)86927-9

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