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Human salivary kallikrein and liberation of colostrokinin



Human salivary kallikrein and liberation of colostrokinin



Proc. Int. Symp. Hypotensive Peptides, Florence, 161-73



Bovine colostrum obtained immediately after calving was centrifuged, the supernatant liquid adjusted to pH 4.6, centrifuged again, and the supernatant liquid or fractions precipitated by salting out with (NH4)2SO4(0.3, 0.5 and fully saturated) were kept at pH 3.0 and 37 degrees C for 5 min and were then adjusted to pH 5.5 and dialysed. Crude colostrokinin was liberated from the non-dialysable protein (crude colostrokininogen) by incubation with human salivary kallikrein.

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Accession: 014509411

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