Indirect fluorescent antibody tests for parasitic diseases. I. Preparation of a stable antigen from larvae of Trichinella spiralis
Journal of Parasitology 51(5): 717-721
ISSN/ISBN: 0022-3395 PMID: 5323264 DOI: 10.2307/3276145
A stable antigen was developed for the indirect fluorescence test for Trichinella spiralis antibodies. The antigen was prepared by digesting fresh larvae of T. spiralis with pepsin for 36 hr and fixing the cuticle in 10% formol-0.5% bovine serum albumin. The antigen can be stored for several months without loss of activity or specificity. Evidence is advanced to support the hypothesis that specific antigenic sites are a constituent part of the larval cuticle.