Section 15
Chapter 14,519

Indirect fluorescent antibody tests for parasitic diseases. I. Preparation of a stable antigen from larvae of Trichinella spiralis

Sulzer, A.J.

Journal of Parasitology 51(5): 717-721


ISSN/ISBN: 0022-3395
PMID: 5323264
DOI: 10.2307/3276145
Accession: 014518804

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A stable antigen was developed for the indirect fluorescence test for Trichinella spiralis antibodies. The antigen was prepared by digesting fresh larvae of T. spiralis with pepsin for 36 hr and fixing the cuticle in 10% formol-0.5% bovine serum albumin. The antigen can be stored for several months without loss of activity or specificity. Evidence is advanced to support the hypothesis that specific antigenic sites are a constituent part of the larval cuticle.