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Karyotype analysis of Sitka Spruce, Picea sitchensis (Bong.) Carr



Karyotype analysis of Sitka Spruce, Picea sitchensis (Bong.) Carr



Silvae Genet, 14: 4, 127-32



Solutions of 1 % colchicine and 0.002M 8-hydroxyquinoline (8-HQ) were used to determine their quantitative effects on spindle inhibition and chromosome contraction. Maximum contraction (50%) was obtained with successive applications of both, but undesirable effects of 8-HQ preclude its use in karyotype analysis. Colchicine caused complete inhibition of the spindle and contracted chromosomes by ca. 40%. There was no differential contraction between chromosomes or between arms.

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Accession: 014540958

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