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Serological studies on the insemination of domestic fowl



Serological studies on the insemination of domestic fowl



Jap Poultry Sci 1(1): 33-38



A few reports have indicated that antibodies against spermatozoa formed in domestic animals by the application of artificial insemination have detrimental effects on the subsequent fertility. The present study was undertaken to see both the serological nature of chicken spermatozoa and of agglutinins against chicken spermatozoa in the sera of virgin pullets and inseminated hens. The results obtained are as follows; isoantibodies against chicken spermatozoa were produced by intravenous injection in domestic fowls; agglutinogens of chicken spermatozoa were impaired by heating at 56 [degree]C for 15[image], or by keeping at room temperature or refrigerator for over -night, however, the absorbing properties of such treated spermatozoa to the agglutinins were not influenced by the treatments; no individual and breed (WL, WR and NH) differences of agglutinogens of chicken spermatozoa were found; normal isoagglutinins against the chicken spermatozoa were found in the sera of 103 day-old chickens; The agglutinin titers in the sera of adult chickens were relatively constant for over 2 months.

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