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Use Of Free Amino Acid Concentrations In Blood Plasma In Evaluating The Amino Acid Adequacy Of Intact Proteins For Chick Growth. I. Free Amino Acid Patterns Of Blood Plasma Of Chicks Fed Unheated And Heated Fishmeal Proteins



Use Of Free Amino Acid Concentrations In Blood Plasma In Evaluating The Amino Acid Adequacy Of Intact Proteins For Chick Growth. I. Free Amino Acid Patterns Of Blood Plasma Of Chicks Fed Unheated And Heated Fishmeal Proteins



J. Nutrition. 86: 37-44



New Hampshire x Columbian cockerels were fed on a practical maize and soya ration for the first 7 days of life and then for 4 days on a purified diet based on isolated soya protein and glucose. Groups of 10 were selected on the basis of weight and fed on a standard reference diet containing crystalline amino acids for 3 days during which they were trained to interrupted feeding, and all received a similar amino acid intake. After a 4-h fast they were given experimental diets in which heated and unheated fishmeals replaced the pure amino acid mixture. Feeding was at the rate of 0.8 g per bird every 30 min for 12 feedings, and 30 min after the last feeding blood samples were withdrawn by heart puncture. The plasma from 8 birds in each group was pooled, deproteinised with picric acid and subjected to amino acid analysis on a Beckman automatic analyser. Except for lysine the concentrations of essential amino acids were lower for the fishmeal groups than for those on the corresponding standard reference diet, and the amino acids which appeared to be most deficient in the fishmeals were histidine, valine, threonine and methionine. Mild over-heating caused lower plasma concentrations of lysine and threonine and more drastic overheating (12 h autoclaving at 121o) reduced the concentrations of all amino acids.

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