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31P nuclear magnetic resonance analysis of Penicillium ochro-chloron



31P nuclear magnetic resonance analysis of Penicillium ochro-chloron



Biochemical and Biophysical Research Communications 106(2): 596-601



31P nuclear magnetic resonance (NMR) spectra of a filamentous fungus P.ochro-chloron were obtained at a frequency of 40.3 MHz. The spectra show resonances assignable to sugar phosphates, orthophosphates, glycerol-3-phosphorylethanolamine (GPE), glycerol-3-phosphorylcholine (GPC), ATP-, UDPG and polyphosphate. The intracellular pH is estimated to be 6.4 in the logarithmic phase of growth, and high levels of GPE and GPC are characteristic of the fungus. The 31P NMR spectra reflect the age of the fungus and show the accumulation of polyphosphate in the stationary phase.

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Accession: 014828323

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PMID: 7104010

DOI: 10.1016/0006-291x(82)91152-4


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