+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A Wnt3a variant participates in chick apical ectodermal ridge formation: distinct biological activities of Wnt3a splice variants in chick limb development



A Wnt3a variant participates in chick apical ectodermal ridge formation: distinct biological activities of Wnt3a splice variants in chick limb development



Development Growth and Differentiation 49(6): 493-501



Wnt/beta-catenin signaling is involved in the formation of the apical ectodermal ridge (AER) during vertebrate limb development. Although Wnt3a is a potent ligand for chick AER formation, whether chick Wnt3a can induce Fgf8 expression in chick embryos is unclear and the Wnt ligand involved in chick AER formation remains unknown. Here, we examined whether another Wnt3a isoform is expressed in the AER, and whether Wnt3 contributes to AER formation in chick as well as mouse embryos. We found that chick Wnt3 was not expressed in the presumptive limb ectoderm at the early stages of AER formation. Using 5'-rapid amplification of cDNA ends, we isolated another chick Wnt3a transcript. This novel variant, Wnt3a variant 2, induced Fgf8 in the limb ectoderm and activated the beta-catenin pathway in vivo and in vitro. These data showed that Wnt3a variant 2 is an active form of chick Wnt3a that regulates chick AER formation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 014836498

Download citation: RISBibTeXText

PMID: 17488271

DOI: 10.1111/j.1440-169x.2007.00938.x


Related references

Form and structure of the apical ectodermal ridge during chick limb development. European Journal of Cell Biology 24(2): 332, 1981

FGF-2: apical ectodermal ridge growth signal for chick limb development. Science 264(5155): 104-107, 1994

Formation of a functional apical ectodermal ridge from nonridge ectoderm in limb buds of chick and quail embryos. Journal of Experimental Zoology 226(2): 221-230, 1983

Formation of a functional apical ectodermal ridge from nonlimb ectoderm in limb buds of chick and quail embryos. Anatomical Record 193(3): 631, 1979

Dorso-ventral ectodermal compartments and origin of apical ectodermal ridge in developing chick limb. Development 124(22): 4547-4556, 1997

Msx1 expressing mesoderm is important for the apical ectodermal ridge (AER)-signal transfer in chick limb development. Development Growth and Differentiation 39(6): 705-714, 1997

Msx1 expressing mesoderm is important for the apical ectodermal ridge (AER)-signal transfer in chick limb development. Development Growth and Differentiation 39(6): 705-714, 1997

Regeneration Of The Apical Ectodermal Ridge Of The Chick Limb Bud. Developmental Biology 9: 38-55, 1964

A splice variant of CD44 expressed in the apical ectodermal ridge presents fibroblast growth factors to limb mesenchyme and is required for limb outgrowth. Genes & Development 12(7): 1058-1071, 1998

A splice variant of CD44 expressed in the rat apical ectodermal ridge contributes to limb outgrowth. Annals of the new York Academy of Sciences 785: 345-349, 1996

Glycosamino glycan synthesis by the apical ectodermal ridge of chick limb bud. Nature (London) 291(5812): 231-232, 1981

Effects of the apical ectodermal ridge on growth of the versene-stripped chick limb bud. Developmental Biology 3: 1-25, 1961

Apical ectodermal ridge induction by the transplantation of En-1-overexpressing ectoderm in chick limb bud. Development Growth & Differentiation. 40(4): 423-429,., 1998

Influence of the apical ectodermal ridge on the proximo distal gradient of chick limb bud myo blasts. Journal of Cell Biology 91(2 Part 2): 25A, 1981

Involution in vitro, regeneration and functional activity of the apical ectodermal ridge of the chick limb bud. Anatomia Histologia Embryologia 8(3): 262-276, 1979