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A comparative study of the phospholipid profiles of guinea pig cardiac muscle and bullfrog cardiac and thigh skeletal muscle, and their in-vitro differential deacylation by endogenous phospholipases. Thin layer chromatographic and densitometric analysis



A comparative study of the phospholipid profiles of guinea pig cardiac muscle and bullfrog cardiac and thigh skeletal muscle, and their in-vitro differential deacylation by endogenous phospholipases. Thin layer chromatographic and densitometric analysis



Journal of Planar Chromatography Modern TLC 20(3): 209-215



The phospholipid profiles of guinea pig and bullfrog cardiac muscle and of bullfrog thigh skeletal muscle before and after in-vitro treatment with endogenous phospholipases at pH 7.4 and 38 degrees C for 60 min have been analyzed by densitometric thin-layer chromatography (TLC). The results revealed:a noticeably high level of cardiolipin (CL) in guinea pig heart which was greatly reduced by in-vitro incubation, with concurrent production of monolysocardiolipin (MLCL);the amount of CL was much lower in bullfrog cardiac muscle than in guinea pig heart, and a small amount of MLCL was produced during in-vitro incubation;the amount of CL was lowest in bullfrog thigh muscle and no MLCL was produced by in-vitro incubation;guinea pig cardiac muscle contains both phosphatidyl ethanol-amine (PE) and phosphatidyl choline (PC) plasmalogens;PE plasmalogen is the only alkenyl species present in bullfrog cardiac muscle and thigh skeletal muscle; andno lyso plasmalogen was produced during in-vitro incubation of these three organs.Some sense of order is emerging in the lipolytic action of these tissues on their endogenous phospholipids. This is probably related to the function of these tissues in vivo.

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