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An ATP-dependent deoxyribonuclease from Diplococcus pneumoniae. II. Evidence for its involvement in bacterial recombination



An ATP-dependent deoxyribonuclease from Diplococcus pneumoniae. II. Evidence for its involvement in bacterial recombination



Biochimica et Biophysica Acta 224(1): 42-54



1.1. Mutants of Diplococcus pneumoniae sensitive to -radiation were isolated as a means of selecting recombination-deficient strains.2.2. One -radiation-sensitive strain, A5-5–9A, which was found to be deficient in recombination, also lacked the ATP-dependent deoxyribonuclease activity and exhibited an increased sensitivity to ultraviolet radiation.3.3. Genetic evidence is presented to demonstrate that all four phenotypes of A5-5–9A (increased sensitivity to ultraviolet and -radiation, deficiency in recombination, and loss of the ATP-dependent deoxyribonuclease activity) transfer as a single marker in transformation, a fact which shows that the defect in the ATP-dependent deoxyribonuclease activity is presumably responsible for the increased radiation sensitivity as well as the decreased recombination proficiency.4.4. The enzyme is apparently not essential for the process whereby different genetic pneumococcal markers are known to transform with markedly different efficiencies: the transformation frequency of the nuclease deficient mutant is decreased by the same factor for both the “high efficiency” ery-r 2 and “the low efficiency” opt-r 2 marker.

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Accession: 015007681

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PMID: 4395244

DOI: 10.1016/0005-2787(70)90618-0


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