+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Analysis of mismatched DNA by mismatch binding ligand (MBL)-Sepharose affinity chromatography

, : Analysis of mismatched DNA by mismatch binding ligand (MBL)-Sepharose affinity chromatography. Analytical and Bioanalytical Chemistry 388(5-6): 1165-1173

Mismatch binding molecules (MBLs), strongly and selectively bound to the mismatched base pair in duplex DNA, were immobilized on Sepharose. Three MBL-Sepharose columns were prepared with three MBLs, naphthyridine dimer (ND), naphthyridine-azaquinolone (NA), and aminonaphthyridine dimer (amND), which exhibited different binding profiles to the mismatched base pairs. These three MBL-Sepharose columns showed characteristic elution profiles for DNA duplexes containing mismatched base pairs. The ND-Sepharose column separated the G-G and G-A mismatched DNA from fully matched duplexes. The NA-Sepharose column separated the A-A and G-A mismatched DNA from other DNA duplexes. The amND-Sepharose column separated the C-C mismatched DNA. These chromatographic profiles were very consistent with the binding preference of each MBL. By changing the elution conditions from sodium hydroxide to sodium chloride, MBL-Sepharose columns were also able to separate the mismatched DNA that weakly bound to the MBL from fully matched DNA duplex. Figure MBL-Sepharose affinity chromatography successfully separates the mismatched duplex DNA from fully matched duplex.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 015034441

PMID: 17541568

DOI: 10.1007/s00216-007-1323-y

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Nagasawa, Takeshi; Takeda, Teiji; Minemura, Kesami; Degroot, Leslie J., 1997: Oct-1, silencer sequence, and GC box regulate thyroid hormone receptor beta-1 promoter. Thyroid hormone, acting through thyroid hormone receptors (TRs), plays a crucial role in brain development and its insufficiency results in irreversible brain damage. TRa mRNA is expressed continuously from early embryonic stages, but the level of...

Romien, M., 1949: L'hypophyse du gecko (Tarentola mauritanica L.). CR Ass Anat, 55: 334-341

Pocock, R. I., 1901: On some new Trap-door Spiders from China. Proceedings of the Zoological Society, (i) 207-215

Di Tommaso, Annalisa; Saletti, Giulietta; Pizza, Mariagrazia; Rappuoli, Rino; Dougan, Gordon; Brignani, Sergio; Douce, Gill; De Magistris, Maria Teresa, 1996: Induction of antigen-specific antibodies in vaginal secretions by using a nontoxic mutant of heat-labile enterotoxin as a mucosal adjuvant. Immunization of the female reproductive tract is important for protection against sexually transmitted diseases and other pathogens of the reproductive tract. However, intravaginal immunization with soluble antigens generally does not induce high...

Schley, P., 1968: On the use of artificially produced ion densities in animal houses. A review of Soviet publications confirming the favourable effects of air anions on production characters of various species of livestock and poultry.

Parodi, Aj, 1981: Biosynthetic mechanisms for cell envelope polysaccharides Yeasts, glucan, chitin, mannan. Yeast cell envelopes: biochemistry biophysics and ultrastructure editor Wilfred Niels Arnold: 4

Yang, Pinfen; Sale, Winfield S., 1999: Flagellar radial spoke protein 2 is a protein kinase. Molecular Biology of the Cell 10(SUPPL ): 388a, Nov

Chi, J-Tsan.; Chang, H.Y.; Haraldsen, G.; Jahnsen, F.L.; Troyanskaya, O.G.; Chang, D.S.; Wang, Z.; Rockson, S.G.; van de Rijn, M.; Botstein, D.; Brown, P.O., 2003: Endothelial cell diversity revealed by global expression profiling. The vascular system is locally specialized to accommodate widely varying blood flow and pressure and the distinct needs of individual tissues. The endothelial cells (ECs) that line the lumens of blood and lymphatic vessels play an integral role in...

Klein, I.; Klootwijk, J., 1976: Determination of 2 o methyl groups in phosphorus 32 labeled rna. A 2-dimensional method for the detection of ribose methyl groups in 32P-labeled RNA is described which is suitable for both qualitative and quantitative purposes. The method involves fractionation of an alkaline hydrolysate of the RNA by 2-dimensi...

Hansen, F.; Hammerum, A.M.; Skov, R.L.; Giske, C.G.; Sundsfjord, A.; Samuelsen, O., 2013: Evaluation of ROSCO Neo-Sensitabs for phenotypic detection and subgrouping of ESBL-, AmpC- and carbapenemase-producing Enterobacteriaceae. The increasing prevalence of ESBL-, AmpC- and carbapenemase-producing Enterobacteriaceae necessitates reliable phenotypic tests for detection and categorization. The main objective of this study was to evaluate ROSCO Neo-Sensitabs with different ...