Carotenoid synthesis and pleiotropic effects in carotenoid-deficient seedlings of maize

Mayfield, S.P.; Nelson, T.; Taylor, W.C.; Malkin, R.

Planta 169(1): 23-32


ISSN/ISBN: 0032-0935
PMID: 24232425
DOI: 10.1007/bf01369771
Accession: 015214616

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Plastid-envelope membranes from seedlings ofZea mays L. made carotenoid-deficient by either norflurazon treatment or mutation lack an activity permitting conversion of phytoene to β-carotene. This activity in membrane fractions was measured by coincubation in vitro with a soluble system from spinach chloroplasts capable of converting [(14)C]isopentenyl pyrophosphate into phytoene. When grown in light, the carotenoid-deficient seedlings lack many soluble chloroplast proteins, including NADP-dependent malic enzyme (EC, pyruvate phosphate dikinase (EC, and ribulose-1,5-bisphosphate carboxylase (EC, but apparently still contain the soluble activities permitting synthesis of phytoene.