+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cell selective glucocorticoid induction of caveolin-1 and caveolae in differentiating pulmonary alveolar epithelial cell cultures



Cell selective glucocorticoid induction of caveolin-1 and caveolae in differentiating pulmonary alveolar epithelial cell cultures



Biochemical and Biophysical Research Communications 359(2): 360-366



Increased caveolin-1 expression is a marker of the differentiation of lung alveolar epithelial type II cells into a type I phenotype. Here, we show in both a primary differentiating rat alveolar culture, and a human alveolar cell line (A549) that caveolae formation and caveolin-1 expression are dependent upon dexamethasone Dex, and is inhibited by the glucocorticoid receptor (GR) antagonist, mifepristone. Study of a panel of 20 different cell types showed the effect of (Dex) upon caveolin-1 expression to be highly cell selective for lung alveolar epithelial cells. The actions of glucocorticoid upon caveolin-1 appear indirect acting via intermediary genes as evidenced by cycloheximide (CHX) abolition of Dex-induced increases in caveolin-1 mRNA and by recombinant transfection studies using the caveolin-1 promoter cloned upstream of a reporter gene. Treatment with actinomycin D (ACD) revealed that the effects of Dex are also, at least in part, mediated by stabilisation of caveolin-1 mRNA. Collectively, these results indicate that glucocorticoids modulate the expression of caveolin-1 and caveolae biogenesis within alveolar epithelial cells via both transcriptional and translational modifications. The cell-selective effects of glucocorticoid upon caveolin may represent a previously unrecognised mechanism by which glucocorticoids affect lung development.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 015224431

Download citation: RISBibTeXText

PMID: 17537407

DOI: 10.1016/j.bbrc.2007.05.106


Related references

Caveolin-1 expression and caveolae biogenesis during cell transdifferentiation in lung alveolar epithelial primary cultures. Biochemical and Biophysical Research Communications 262(3): 744-751, 1999

Caveolin and its cellular and subcellular immunolocalisation in lung alveolar epithelium: Implications for alveolar epithelial type I cell function. Cell & Tissue Research 295(1): 111-120, 1999

Cell surface orifices of caveolae and localization of caveolin to the necks of caveolae in adipocytes. Molecular Biology of the Cell 14(10): 3967-3976, 2003

Increased smooth muscle cell expression of caveolin-1 and caveolae contribute to the pathophysiology of idiopathic pulmonary arterial hypertension. Faseb Journal 21(11): 2970-2979, 2007

The cytochrome p 450 monooxygenase system of rabbit lung enzyme components activities and induction in the nonciliated bronchiolar epithelial clara cell alveolar type ii cell and alveolar macrophage. Molecular Pharmacology 30(3): 296-303, 1986

A new rat type I-like alveolar epithelial cell line R3/1: bleomycin effects on caveolin expression. Histochemistry and Cell Biology 121(6): 509-519, 2004

Caveolae mediated albumin transport in cultures of alveolar type I-like epithelial monolayers. Journal of Pharmacy & Pharmacology 51(SUPPL ): 132, Sept, 1999

Cell-cell communication in heterocellular cultures of alveolar epithelial cells. American Journal of Respiratory Cell and Molecular Biology 29(5): 552-561, 2003

Temporal changes in caveolin expression in cultures alveolar type II cell monolayers Potential significance to in-vitro transport studies. European Journal of Pharmaceutical Sciences 4(SUPPL ): S194, 1996

Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons: Caveolin-2 is up-regulated in response to cell injury. Proceedings of the National Academy of Sciences of the United States of America 95(17): 10257-10262, 1998

Cell biology of caveolae and caveolin. Advanced Drug Delivery Reviews 49(3): 223-235, 2001

Regulation of rat pulmonary dendritic cell immunostimulatory activity by alveolar epithelial cell-derived GM-CSF. Clinical Research 42(3): 397A, 1994

Caveolin, caveolae, and endothelial cell function. Arteriosclerosis, Thrombosis, and Vascular Biology 23(7): 1161-1168, 2003

Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis. Respiratory Research 19(1): 175, 2018

Expression of a differentiation related cytokeratin is modulated by cell cell contacts in pulmonary alveolar epithelial cells. Journal of Cell Biology 115(3 PART 2): 398A, 1991