+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cloning and sequence analysis of the spike gene from several feline coronaviruses



Cloning and sequence analysis of the spike gene from several feline coronaviruses



Advances in Experimental Medicine and Biology 342: 17-21



The DNA sequence encoding the spike gene from the DF2 strain of Type II feline infectious peritonitis virus (FIPV), a temperature sensitive FIPV virus (TS-DF2) and an isolate of feline enteric coronavirus (FECV 1683) were determined. Comparison of the published WSU 1146 and DF2 FIPV S genes showed that the viruses shared a high degree of homology (99.6%). Likewise, the S gene of the virulent DF2 FIPV virus was closely conserved to that isolated from the vaccine virus strain, TS-DF2 FIPV. In contrast, the FECV S gene had numerous DNA and amino acid differences when compared to the virulent FIPV sequences. Sequence differences among the feline coronavirus isolates were localized to the amino-terminus region of the S gene.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 015292083

Download citation: RISBibTeXText

PMID: 8209725


Related references

Nucleotide sequence and expression of the spike (S) gene of canine coronavirus and comparison with the S proteins of feline and porcine coronaviruses. Journal of General Virology 75: 1789-1794, 1994

Nucleotide sequence and expression of the spike (S) gene of canine coronavirus and comparison with S proteins of feline and porcine coronaviruses. Journal of General Virology 75(7): 1789-1794, 1994

Sequence analysis of the nucleocapsid gene of feline coronaviruses circulating in Italy. New Microbiologica 33(4): 387-392, 2010

Genomic analysis of 16 Colorado human NL63 coronaviruses identifies a new genotype, high sequence diversity in the N-terminal domain of the spike gene and evidence of recombination. Journal of General Virology 93(Pt 11): 2387-2398, 2012

Cloning and sequence analysis of spike protein gene of canine coronavirus strain V1. Journal of Shanghai Jiaotong University Agricultural Science 22(3): 266-271, 2004

Sequence Analysis of Feline Coronaviruses and the Circulating Virulent/Avirulent Theory. Emerging Infectious Diseases 17(4): 744-746, 2011

Sequence analysis of feline coronaviruses and the circulating virulent/avirulent theory. Emerging Infectious Diseases 17(4): 744-746, 2011

Molecular cloning and sequence analysis of feline interferon-stimulated gene 15. Veterinary Immunology and Immunopathology 126(1-2): 20-26, 2008

Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99. Virus Genes 26(3): 239-246, 2003

Cloning and sequence analysis of spike protein gene of swine transmissible gastroenteritis coronavirus. Journal of Northwest A and F University Natural Science Edition 35(7): 1-6, 2007

Cloning and sequence analysis of spike protein gene of canine coronavirus giant panda's isolate. Virologica Sinica 19(5): 481-486, 2004

Cloning and further sequence analysis of the spike gene of attenuated porcine epidemic diarrhea virus DR13. Virus Genes 35(1): 55-64, 2007

Cloning, sequence analysis and expression of B and C antigenic sites of TGEV spike gene in prokaryotic system. Chinese Journal of Veterinary Medicine 41(2): 3-6, 2005

Cloning and sequence analysis of partial spike gene 5' region of transmissible gastroenteritis coronavirus TH-98 strain. Chinese Journal of Veterinary Medicine 40(4): 3-7, 2004

The molecular genetics of feline coronaviruses: comparative sequence analysis of the ORF7a/7b transcription unit of different biotypes. Virology 212(2): 622-631, 1995