Cloning the cDNA fragments of differentially expressed genes between seeded and seedless fruit of seedless litchi

Xuan WeiYan; Zheng XueQin

Guangxi Zhiwu / Guihaia 26(6): 597-601

2006


Accession: 015292518

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Abstract
The suppression subtractive hybridization technique was used to clone the cDNA fragments of differentially expressed genes between seeded and seedless fruits in Litchi chinensis cv. Hainan seedless. One positive differential expressing cDNA library was constructed using the cDNA of seeded fruits as driver and the cDNA of seedless fruits as tester. A total of 61 positive clones were obtained by using the reverse Northern Dot-Blot to screen the cDNA library. Nucleotide sequences of 17 randomly chosen positive clones were sequenced and 10 were different sequences. Subsequent sequence analysis showed that the 6 clones corresponding to their nucleotide sequences were thought to be first reports in L. chinensis cultivars.