+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Conjugated linoleic acid reduces adipose depots without reduction of stearoyl coenzyme A desaturase 1 gene expression



Conjugated linoleic acid reduces adipose depots without reduction of stearoyl coenzyme A desaturase 1 gene expression



Annals of Nutrition and Metabolism 51(2): 126-133



This study investigated the effects of conjugated linoleic acid (CLA) on the body weight, fat deposition and the expression of stearoyl coenzyme A desaturase 1 (SCD1) in the livers of male ICR mice that were fed with either beef tallow (BT) or fish oil (FO) supplemented with CLA. Mice weighing 25-30 g were divided into four groups, BT and BT supplemented with 0.5% CLA (BTC), FO and FO supplemented with 0.5% CLA (FOC). Each group consisted of 8 mice, and they were fed the experimental diets for 4 weeks. The experimental diets were composed of 59.18% carbohydrates, 19.73% proteins and 21.09% fat in terms of their contributions to total calories, and other nutrients were identical. CLA was added to the diets of the CLA supplementation groups at 0.5% (w/w). The measurement of triglyceride (TG) was done by using a kit. Fatty acid compositions were analyzed in both the plasma and the liver using a gas chromatograph. The levels of SCD1 expression were analyzed by RT-PCR in the liver. No significant difference was found in the levels of food intake, body weight and food efficiency among experimental groups. However, the levels of both epididymal and visceral fat weight were significantly lower in the CLA-supplemented groups, BTC and FOC (p < 0.05). The level of the triglyceride concentration was also significantly lower in the CLA-supplemented groups (p < 0.05). The levels of the liver SCD1 gene expression were also higher in the CLA-supplemented groups. However, significant inhibition of conversion from C16:0 to C16:1 was found in both fatty acid compositions of the liver (p < 0.05). Addition of 0.5% CLA did not inhibit the liver SCD1 gene expression; however, it showed decreasing effects on the fat pad weight, the concentration of TG and on fatty acid composition, leading to a decrease in the fat depositions. Such effects were clearer when CLA was supplemented to BT rather than FO.

(PDF emailed within 0-6 h: $19.90)

Accession: 015351880

Download citation: RISBibTeXText

PMID: 17536189

DOI: 10.1159/000103272


Related references

Conjugated linoleic acid depresses the D9 desaturase index and stearoyl coenzyme A desaturase enzyme activity in porcine subcutaneous adipose tissue. Journal of Animal Science 80(8): 10-15, 2002

Conjugated linoleic acid depresses the delta9 desaturase index and stearoyl coenzyme A desaturase enzyme activity in porcine subcutaneous adipose tissue. Journal of Animal Science 80(8): 2110-2115, 2002

Effect of dietary conjugated linoleic acid on the fatty acid composition of egg yolk, plasma and liver as well as hepatic stearoyl-coenzyme A desaturase activity and gene expression in laying hens. Poultry science 84(12): 1886-1892, 2005

Transcriptional analysis reveals a high impact of conjugated linoleic acid on stearoyl-Coenzyme A desaturase 1 mRNA expression in mice gastrocnemius muscle. Genes and Nutrition 7(4): 537-548, 2012

The trans-10,cis-12 isomer of conjugated linoleic acid downregulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. Journal of Nutrition 130(8): 1920-1924, 2000

Effect of conjugated linoleic acid on bovine mammary cell growth, apoptosis and stearoyl Co-A desaturase gene expression. Domestic Animal Endocrinology 34(3): 284-292, 2007

Regulation of stearoyl-coenzyme A desaturase and fatty acid delta-6 desaturase-2 expression by linoleic acid and arachidonic acid in human sebocytes leads to enhancement of proinflammatory activity but does not affect lipogenesis. British Journal of Dermatology 165(2): 269-276, 2011

Trans10, cis12 conjugated linoleic acid regulates stearoyl-coenzyme A desaturase 1 through SREBP1 in primary goat mammary epithelial cells. Journal of Agricultural and Food Chemistry 2019, 2019

Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochemical and Biophysical Research Communications 248(3): 817-821, 1998

Subcutaneous and intramuscular adipose tissue stearoyl-coenzyme A desaturase gene expression and fatty acid composition in calf- and yearling-fed Angus steers. Journal of Animal Science 89(8): 2556-2570, 2011

Dietary whole and cracked linseed increases the proportion of oleic and α-linolenic acids in adipose tissues and decreases stearoyl-coenzyme A desaturase, acetyl-coenzyme A carboxylase, and fatty acid synthase gene expression in the longissimus thoracis muscle of Yanbian Yellow cattle. Journal of Animal Science 95(2): 718-726, 2017

The effect of conjugated linoleic acid (CLA) on transcriptional activation of the Stearoyl-CoA desaturase gene in bovine mammary cells. Journal of Dairy Science 88: 288-288, 2005

Characterization of the liver X receptor-dependent regulatory mechanism of goat stearoyl-coenzyme A desaturase 1 gene by linoleic acid. Journal of Dairy Science 99(5): 3945-3957, 2017

Stearoyl-coenzyme A desaturase gene expression during growth in adipose tissue from obese and crossbred pigs. Journal of Animal Science 77(7): 1710-1716, 1999

Postnatal development of stearoyl coenzyme A desaturase gene expression and adiposity in bovine subcutaneous adipose tissue. Journal of Animal Science 77(3): 630-636, 1999