Section 16
Chapter 15,359

Construction of a vector expressing melon PG anti-sense gene and its transformation to Nicotiana tabacum

Huang YongHong; Mei Mei; Zeng JiWu; Zhou BiRong; Wu YuanLi; Yi GanJun

Journal of Fruit Science 24(4): 492-495


Accession: 015358251

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A donor plasmid pBI-aPG was digested with restriction endonucleases; the acquired target gene was ligated to recipient plasmid pCAMBIA2301, which was digested with the same restriction endonucleases; and finally, a plant expression vector named pCB-aPG expressing melon PG antisens gene, GUS gene and NPT II gene was constructed. Moreover, pCB-aPG was transferred into Agrobacterium tumefaciens LBA4404 by direct DNA transfer.

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