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Cryopreservation of a myovirus infecting the toxin-producing cyanobacterium Microcystis aeruginosa



Cryopreservation of a myovirus infecting the toxin-producing cyanobacterium Microcystis aeruginosa



Microbes and Environments 22(3): 297-299



We optimized conditions for cryopreservation of a cyanophage (Ma-LMM01) infecting the toxic cyanobacterium Microcystis aeruginosa. The quality of cryopreservation was estimated by comparing the phage titer before and after preservation at 4, -80, or -196 degrees C and with or without a cryoprotectant: glycerol, dimethyl sulfoxide (DMSO), or Cellbanker. Storage at 4 degrees C for 100 days resulted in similar to 90% loss of infectivity; whereas cryopreservation at -196 degrees C resulted in stable preservation with or without cryoprotectant. Therefore, we established methods to stably preserve the phage, Ma-LMM01, that may be useful in further studies of cyanophages and may be used in isolating new phages.

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Accession: 015413560

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DOI: 10.1264/jsme2.22.297


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